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          現在位置首頁>實驗試劑制品>生化試劑>糖類>Midori Green Advance Agarose Tablets
          Midori Green Advance Agarose Tablets
          Midori Green Advance Agarose Tablets
          • Midori Green Advance Agarose Tablets

          Midori Green Advance Agarose Tablets

          產品報價:詢價

          更新時間:2022/4/13 16:40:33

          地:美國

          牌:Bulldog-Bio

          號:Midori

          廠商性質: 生產型,貿易型,服務型,

          公司名稱: 世聯博研(北京)科技有限公司

          產品關鍵詞: Midori Green Advance Agarose T   Midori Green Advance Agarose T   Midori Green Advance Agarose T  

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          Don’t waste time pouring gels
          Midori Green Advance Agarose Tablets are a fast, clean solution for preparing agarose gels without any additional time-consuming steps, such as weighing or adding different components. Just add the tablet to pure cold water, heat, and pour. That’s it! Once the gel hardens, it’s ready ready for loading. Each tablet contains the perfect amount of Midori Green Advance DNA Stain, with either no buffer, TAE powder or TBE powder, and 0.5g of the highest-purity agarose as integrated components; you don’t need anything else but water. If you’re tired of prepping agarose gels for your lab, this is the quickest and easiest solution to reduce effort and improve the quality of your gels.

          Hey! The stain is already in that little tablet!
          Another big advantage is safety. Midori Green Advance Nucleic Acid Stain is a new, safe alternative to traditional ethidium bromide(EtBr) stain for detecting dsDNA, ssDNA and RNA in agarose gels. Nearly identical to EtBr in performance and use, Midori Green is much less harmful to living organisms. As compared with EtBr, which is known as a strong mutagen, Midori Green causes many fewer mutations in the Ames test. When compared with spontaneous mutations caused by water in the Ames test, Midori Green’s rate was only slightly beyond the standard deviation range. In addition (and in contrast to EtBr), Midori Green scores negative in the mouse bone marrow micronucleus test and in the chromosome aberration test, which indicates that it’s considered non-carcinogenic. And for those who prefer to be safe rather than sorry, Midori Green has been tested for latex/nitrile glove penetration, in which both materials show negative results—even after 6 hours of exposure. Most importantly, Midori Green is not considered hazardous waste, and can be disposed of according to standard laboratory procedures.

          Features:
          – Tablet format – no weighing required
          – Powder free
          – Stable at room temperature when protected from light
          – Only pure water is needed
          – Fast dissolving protocol
          – Non-carcinogenic alternative to ethidium bromide
          – Environmentally safe – disposal with regular laboratory waste

           


          Here you can see a gel which was produced with Midori Green Advance Agarose Tablets. 50 ml of water was added, creating a 1% agarose gel.

          The steps could not be easier.
          1) Add 1 tablet
          2) Add 50 ml of water
          3) Head up in a microwave until melted completely
          4) Cool down the agarose to 60°C
          5) Cast your gel


          Safer for you and better for your subcloning
          By using safe Midori Green dyes and safe Blue/Green LED illumination you can improve your subcloning transformation efficiencies by THREE-FOLD. In this-example, a plasmid vector was double digested with suitable restriction enzymes to create two sticky-ended DNA fragments: the lacZ gene (3,536 bp) and the backbone of the vector (4,318 bp). Equal amounts of digested DNA were electrophoresed on 1% agarose gels. The gels were stained with either ethidium bromide or Midori Green Direct gel stain according to the corresponding manuals, and then viewed using either a UV transilluminator or the FastGene Blue/Green LED Illuminator, respectively. The two DNA fragments were excised from the gels and purified using a silica membrane based purification kit. The lacZ gene and the vector backbone were religated using T4 DNA ligase transformed into DH5a cells and plated onto selection plates. The total number of blue and white colonies was counted to evaluate cloning efficiency. Each experiment was conducted in triplicate, and the average cloning efficiency was determined. Midori Green Direct resulted in dramatic increase of positive transformants.

          Midori Green Can Boost Your Cloning Results!

          Ethidium bromide is typically used in conjunction with a strong UV light source to excise DNA bands for purification prior to the ligation reaction. Short wave-length light is known to cause thymidine dimers and damage the DNA. The extent of this damage is not always appreciated. High energy light wreaks havoc on a DNA fragment in mere seconds. As can be seen below, after only a 15 sec exposure of DNA in a standard agarose gel, cloning efficiency starts to drop. And after a 30 sec exposure your cloning experiment is all but dead! In contrast, the cloning efficiency of protocols that use blue or blue/green LEDs are completely unaffected by this deleterious effect. If your lab isn’t able to break itself of its ethidium bromide habit, using a Blue/Green LED illuminator (or imaging system) should have an immediate positive impact on DNA integrity and cloning efficiency.


          UV Transilluminators Kill Cloning Experiments

          Just as sensitive as EtBr
          Both dyes can stain single- and double-stranded nucleic acids with sensitivity and protocols similar to that of EtBr. This makes it a no-brainer as a drop-in replacement. Best yet, not only can you use your existing UV transilluminator and filter sets, Midori Green also works well when matched withBlue LED Gel Illuminators. These harmless light tables are the perfect complement for these stains, as you can work shield-less and goggle-less when excising bands or imaging migration. And because blue light does not cross-link DNA, there is never a worry about damaging DNA during cloning and photographing sessions.



          不要浪費時間澆注凝膠
          Midori Green Advance瓊脂糖片劑是一種快速,清潔的解決方案,可用于制備瓊脂糖凝膠,而無需任何其他費時的步驟,例如稱重或添加不同的成分。只需將片劑加入純凈的冷水中,加熱,然后倒入即可。而已!凝膠硬化后,就可以加載了。每片含有適量的  Midori Green Advance DNA染色劑,不含緩沖劑,TAE粉末或TBE粉末,以及0.5g最高純度的瓊脂糖作為集成成分;除了水,您不需要其他任何東西。如果您厭倦了為實驗室準備瓊脂糖凝膠,這是減少工作量并提高凝膠質量的最快,最簡單的解決方案。

          嘿! 污漬已經在那個小平板電腦中了!
          另一個大優點是安全性。Midori Green Advance核酸染色劑是用于檢測 瓊脂糖凝膠中dsDNA,ssDNA和RNA的傳統溴化乙錠(EtBr)染色劑的一種新型安全替代品。Midori Green在性能和用途上與EtBr幾乎相同,對生物的危害則小得多。與被稱為強誘變劑的EtBr相比,Midori Green在Ames測試中引起的突變更少  。與Ames測試中由水引起的自發突變相比,Midori Green的比率僅略微超出標準偏差范圍。此外(與EtBr相比),綠豆綠在  小鼠骨髓微核試驗  和 染色體畸變  測試,表明它被認為沒有致癌性。對于喜歡安全而不是后悔的人,Midori Green經過了乳膠/丁腈手套滲透性測試,即使暴露6小時,兩種材料仍顯示陰性結果。最重要的是,Midori Green不被視為危險廢物,可以按照標準實驗室程序進行處置。

          特點:
          –片劑形式–無需稱量–無
          粉末
          –避光保護在室溫下穩定
          –僅需純水
          –快速溶解方案
          –溴化乙錠的非致癌替代物
          –環保–定期處理實驗室廢物進行處置

           


          在這里,您可以看到由Midori Green Advance瓊脂糖片制成的凝膠。加入50ml水,產生1%瓊脂糖凝膠。

          步驟再簡單不過了。
          1)加1片
          2)加50毫升水
          3)用微波爐加熱直至完全融化
          4)將瓊脂糖冷卻至60°C 
          5)澆鑄凝膠


          對您而言更安全,對亞克隆更好
          通過使用安全的Midori Green染料和安全的Blue / Green LED照明,您可以通過三倍提高亞克隆轉化效率。在該實施例中,質粒載體用合適的限制酶雙重消化以產生兩個粘性末端DNA片段:lacZ基因(3,536bp)和載體主鏈(4,318bp)。在1%瓊脂糖凝膠上電泳等量的消化DNA。根據相應的手冊,將凝膠用溴化乙錠或Midori Green Direct凝膠染料染色,然后分別使用UV透射儀或FastGene藍/綠LED照射儀進行觀察。從凝膠上切下兩個DNA片段,并使用基于二氧化硅膜的純化試劑盒純化。使用轉化到DH5a細胞中的T4 DNA連接酶將lacZ基因和載體主鏈重新連接,并鋪在選擇板上。計算藍色和白色菌落的總數以評估克隆效率。每個實驗一式三份進行,并確定平均克隆效率。Midori Green Direct導致陽性轉化體的急劇增加。

          Midori Green可以提高您的克隆結果!

          溴化乙錠通常與強紫外線光源結合使用,以切除DNA條帶,以便在連接反應之前進行純化。短波長的光會引起胸苷二聚體并破壞DNA。這種損壞的程度并不總是可以理解的。高能光在短短幾秒鐘內對DNA片段造成破壞。如下所示,在標準瓊脂糖凝膠中僅暴露DNA 15秒后,克隆效率開始下降。經過30秒的曝光后,您的克隆實驗幾乎已死!相反,使用藍色或藍色/綠色LED的協議的克隆效率完全不受此有害影響的影響。如果您的實驗室無法擺脫自己的溴化乙錠習慣,請使用藍色/綠色LED照明器 (或成像系統)應立即對DNA完整性和克隆效率產生積極影響。


          紫外線透射儀殺死克隆實驗

          與EtBr一樣敏感
          兩種染料都可以用與EtBr相似的靈敏度和方法對單鏈和雙鏈核酸進行染色。這使它成為了直接的替代品。最好的是,不僅可以使用現有的紫外線透射照明器和濾光片組,而且與藍色LED凝膠照明器搭配使用時,綠綠也可以很好地工作。這些無害的光桌是這些污漬的完美補充,因為在切割樂隊或成像遷移時,您可以無遮罩和無護目鏡地工作。而且,由于藍光不會使DNA交聯,因此不必擔心在克隆和照相過程中會損壞DNA。

           1 kb DNA階梯經Midori Green 
          和EtBr 染色
          用Midori Green和EtBr染色的100bp DNA階梯